Saravanan Kolandaivelu, PhD – Research Assistant Professor
E363, E357, WVU Eye Institute
PO Box 9193
Morgantown, WV 26506-9131
Ophthalmology; Biochemistry; Rockefeller Neuroscience Institute
PhD in Biochemistry, Structural and Molecular Biology, All India Institute of Medical Sciences, New Delhi, India
Howard Hughes Medical Institute, Biochemistry, University of Washington, Seattle, WA
Biochemist, WVU, Morgantown, WV
Research Assistant Professor
The focus of my laboratory research is to identify the mechanism behind biogenesis and/or maintenance in the photoreceptor outer segment. Photoreceptor outer segments are modified cilia filled with highly ordered membrane discs that harbor signaling proteins responsible for the detection of photons. It is very puzzling to understand how the photoreceptor OS is maintained and disc membranes are organized and stacked together, which is fundamental for proper function and stability of photoreceptor neurons.
Using a combination of physiological, biochemical and molecular multi-disciplinary approaches, including gene editing, animal models, and tissues culture, we strive to understand our long term goal the mechanism behind biogenesis and/or maintenance in the photoreceptor outer segment. Additionally, our lab studies the importance of the post-translational lipid modification “palmitoylation” in retinal proteins associated with blinding diseases. Among lipid modifications, palmitoylation is the only reversible lipid modification. The dynamic reversibility controls many cellular functions including protein trafficking, protein stability, and protein-protein interaction. We will apply our knowledge of these regulatory pathways in retinal function to develop a novel therapeutic approach.
- Understanding mechanism behind biogenesis and/ or maintenance of photoreceptor outer segments.
- Study the importance of membrane potential in photoreceptor stability and function.
- Identification of retinal palmitoyl lipid modified proteins and its role.
- Understanding the role of the NAD-biosynthetic enzyme NMNAT1 in retinal function and stability.
Mr. Joe Murphy, MS
Ms. Emily Sechrest, BS
Graduate Student, Biochemistry
Mr. David Sokolov
Undergraduate Honors Student
- Brooks C, Murphy J, Belcastro M, Heller, D,
Kolandaivelu S, Sokolov M (2018).
Farnesylation of the transducin G protein gamma subunit is a prerequisite for
its ciliary targeting in rod photoreceptors.
Front Mol Neurosci, 11:16. PMCID: PMC5787109
- Murphy J,
Kolandaivelu S (2016).
Palmitoylation of progressive rod-cone degeneration (PRCD) regulates protein
stability and localization..
J Biol Chem, 291(44):23036-23046. PMCID: PMC5087724
- CHristiansen JR, Pendse ND,
Kolandaivelu S, Bergo MO, Young SG, Ramamurthy V (2016).
Deficiency of isoprenylcysteine carboxyl methyltransferase (ICMT) leads to progressive
loss of photoreceptor function.
J Neurosci, 36(18):5107-14. PMCID: PMC4854971
- Murphy D,
Kolandaivelu S, Ramamurthy, V, Stoilov P (2016).
Analysis of alternative pre-RNA splicing in the mouse retina using a fluorescent
Methods Mol Biol, 1421:269-86.
- Murphy D, Singh R,
Kolandaivelu S, Ramamurthy V, Stoilov P (2015).
Alternative splicing shapes the phenotype of a mutation in BBS8 to cause nonsyndromic
Mol Cell Biol, 35(10):1860-70. PMCID: PMC4405636
- Singh RK,
Kolandaivelu S, Ramamurthy V (2014).
Early alteration of retinal neurons in Aipl1-/- animals.
Invest Ophthalmol Vis Sci, 55(5):3081-92. PMCID: PMC4034756
- Kolandaivelu S, Ramamurthy V (2014).
AIPL1 protein and its indispensable role in cone photoreceptor function and survival.
Adv Exp Med Biol, 801:43-8.
- Kolandaivelu S, Singh RK, Ramamurthy V (2014).
AIPL1, a protein linked to blindness, is essential for the stability of enzymes
mediating cGMP metabolism in cone photoreceptor cells.
Hum Mol Genet, 23(4):1002-12. PMCID: PMC3900108
- Deng WT, Sakurai K,
Kolandaivelu S, Kolesnikov AV, Dinculescu A, LiJ, Zhu P, Liu X, Pang
J, Chiodo VA, Boye SL, Chang B, Ramamurthy V, Kefalov VJ, Hauswirth WW (2013).
Cone phosphodiesterase-6α’ restores rod function and confers distinct physiological
properties in the rod phosphodiesterase-6β-deficient rd10 mouse.
J Neurosci, 33(29):11745-53. PMCID: PMC3713718
- Majumder A, Pahlberg J, Boyd KK, Kerov V,
Kolandaivelu S, Ramamurthy V, Sampath AP, Artemyev NO (2013).
Transducin translocation contributes to rod survival and enhances synaptic transmission
from rods to biopolar cells.
Proc Natl Acad Sci USA, 110(30:12468-73. PMCID: PMC3725049
- Kolandaivelu S, Chang B, Ramamurthy V (2011).
Rod phosphodiesterase-6 (PDE6) catalytic subunits restore cone function in a
mouse model lacking cone PDE6 catalytic subunit.
J Biol Chem, 286(38):33252-9. PMCID: PMC3190866
- Christiansen JR,
Kolandaivelu S, Bergo MO, Ramamurthy V (2011).
RAS-converting enzyme 1-mediated endoproteolysis is required for trafficking
of rod phosphodiesterase 6 to photoreceptor outer segments.
Proc Natl Acad Sci USA, 108(21):8862-6. PMCID: PMC3102416